In 2022,Wangngae, Sirilak; Chansaenpak, Kantapat; Weeranantanapan, Oratai; Piyanuch, Pornthip; Sumphanapai, Thitima; Yamabhai, Montarop; Noisa, Parinya; Lai, Rung-Yi; Kamkaew, Anyanee published an article in Scientific Reports. The title of the article was 《Effect of morpholine and charge distribution of cyanine dyes on cell internalization and cytotoxicity》.Application In Synthesis of 1-Methylpiperazine The author mentioned the following in the article:
To improve the potency of Heptamethine cyanines (Hcyanines) in cancer research, we designed and synthesized two novel Hcyanines based theranostic probes, IR794-Morph and IR794-Morph-Mpip, to enhance cancer cell internalization and targeting. In acidic conditions that resemble to tumor environment, both IR794 derivatives exhibited broad NIR absorption band (704-794 nm) and fluorescence emission (798-828 nm) that is suitable for deep seated tumor imaging. Moreover, in vitro study revealed that IR794-Morph-Mpip exhibited better cancer targetability towards various cancer cell lines under physiol. and slightly acidic conditions compared to normal cells. IR794-Morph-Mpip was fast internalized into the cancer cells within the first 5 min and mostly localized in lysosomes and mitochondria. In addition, the internalized signal was brighter when the cells were in the hypoxic environment. Furthermore, cellular uptake mechanism of both IR794 dyes, investigated via flow cytometry, revealed that endocytosis through OATPs receptors and clathrin-mediated endocytosis were the main routes. Moreover, IR794-Morph-Mpip, displayed anti-cancer activity towards all tested cancer cell types with IC50 below 7 μM (at 6 h incubation), which is approx. three times lower than that of the normal cells. Therefore, increasing protonated cites in tumor environment of Hcyanines together with incorporating morpholine in the mol. can enhance structure-inherent targeting of these dyes. The results came from multiple reactions, including the reaction of 1-Methylpiperazine(cas: 109-01-3Application In Synthesis of 1-Methylpiperazine)
1-Methylpiperazine(cas: 109-01-3) can be used as mimic template in the preparation of molecularly imprinted microspheres (MIMs). It was also used to prepare the difunctional strong anion-exchange stationary phase from a 1,4-diazacyclohexane derivative..Application In Synthesis of 1-Methylpiperazine
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics