On March 7, 1990, Kishimoto, Shoji; Fujita, Takeshi; Kanamaru, Tsuneo; Folkman, Moses Judah; Ingber, Donald published a patent.COA of Formula: C7H13ClN2O The title of the patent was Preparation of O-acylfumagillols and analogs as angiogenesis inhibitors. And the patent contained the following:
The title compounds [I; R1 = (un)substituted CH:CMe2, CH2CHMe2; R2 = substituted alkanoyl, aroyl, (un)substituted heteroarylcarbonyl, CONH2, alkyl, PhSO2, alkylsulfonyl, H2NSO2, alkoxycarbonyl, PhO2C] were prepared Thus, fumagillol was stirred 2 h at 0° with ClCH2CONCO in CH2Cl2 containing dimethylaminopyridine to give I (R1 = CH:CMe2, R2 = CONHCOCH2Cl) which suppressed B16 mouse melanoma tumor growth to 20% that of controls after 3 wk in mice receiving 30 mg/kg s.c. every other day. The experimental process involved the reaction of 4-Ethyl-piperazine-1-carbonyl chloride(cas: 53788-12-8).COA of Formula: C7H13ClN2O
The Article related to acylfumagillol preparation angiogenesis inhibitor, fumagillol acyl preparation angiogenesis inhibitor, Terpenes and Terpenoids: Sesquiterpenes (C15), Including Ionones and other aspects.COA of Formula: C7H13ClN2O
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics