On June 15, 2014, Nirantar, Saurabh R.; Li, Xiang; Siau, Jia Wei; Ghadessy, Farid J. published an article.COA of Formula: C12H16N2O2 The title of the article was Rapid screening of protein-protein interaction inhibitors using the protease exclusion assay. And the article contained the following:
We have previously developed a sensitive and modular homogenous biosensor system using peptides to detect target ligands. By transposing the basic mechanistic principle of the nuclease protection assay into this biosensor framework, we have developed the protease exclusion (PE) assay which can discern antagonists of protein-protein interactions in a rapid, single-step format. We demonstrate the concept with multiple protein-peptide pairs and validate the method by successfully screening a small mol. library for compounds capable of inhibiting the therapeutically relevant p53-Mdm2 interaction. The Protease Exclusion method adds to the compendium of assays available for rapid analyte detection and is particularly suited for drug screening applications. The experimental process involved the reaction of 1-(4-(4-Hydroxyphenyl)piperazin-1-yl)ethanone(cas: 67914-60-7).COA of Formula: C12H16N2O2
The Article related to protease small mol screening biosensor p53 mdm2 protein, biosensor, high throughput screening, p53–mdm2 interaction, protein–protein interaction, Pharmacology: Structure-Activity and other aspects.COA of Formula: C12H16N2O2
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics