Wilson, Daniel J. published the artcileA continuous fluorescence displacement assay for BioA: An enzyme involved in biotin biosynthesis, Product Details of C20H17ClN4O3, the publication is Analytical Biochemistry (2011), 416(1), 27-38, database is CAplus and MEDLINE.
Cofactor biosynthetic pathways represent a rich source of potential antibiotic targets. The second step in biotin biosynthesis is performed by BioA, a pyridoxal 5′-phosphate (PLP)-dependent enzyme. This enzyme has been confirmed as a candidate target in Mycobacterium tuberculosis; however, the current bioassay used to measure BioA activity is cumbersome and low throughput. Here we describe the design, development, and optimization of a continuous coupled fluorescence displacement assay to measure BioA activity. In this coupled assay, BioD converts the product of the BioA-catalyzed reaction into dethiobiotin, which is subsequently detected by displacement of a fluorescently labeled dethiobiotin probe from streptavidin. The assay was further adapted to a high-throughput screening format and validated against the LOPAC1280 library.
Analytical Biochemistry published new progress about 115687-05-3. 115687-05-3 belongs to piperazines, auxiliary class Epigenetics,Sirtuin, name is (4-Chlorophenyl)(4-(8-nitroquinolin-5-yl)piperazin-1-yl)methanone, and the molecular formula is C6H8N2, Product Details of C20H17ClN4O3.
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https://en.wikipedia.org/wiki/Piperazine,
Piperazines – an overview | ScienceDirect Topics