Identification and characterization of teratogenic chemicals using embryonic stem cells isolated from a wnt/尾-catenin-reporter transgenic mouse line was written by Kugler, Josephine;Kemler, Rolf;Luch, Andreas;Oelgeschlaeger, Michael. And the article was included in Toxicological Sciences in 2016.Recommanded Product: 4-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline This article mentions the following:
Embryonic stem cells (ESCs) are commonly used for the anal. of gene function in embryonic development and provide valuable models for human diseases. In recent years, ESCs have also become an attractive tool for toxicol. testing, in particular for the identification of teratogenic compounds The authors have recently described a Bmp-reporter ESC line as a new tool to identify teratogenic compounds and to characterize the mol. mechanisms mediating embryonic toxicity. Here the authors describe the use of a Wnt/尾-Catenin-reporter ESC line isolated from a previously described mouse line that carries the LacZ reporter gene under the control of a 尾-Catenin responsive promoter. The reporter ESC line stably differentiates into cardiomyocytes within 12 days. The reporter was endogenously induced between day 3-5 of differentiation reminiscent of its expression in vivo, in which strong LacZ activity is detected around gastrulation. Subsequently its expression becomes restricted to mesodermal cells and cells undergoing an epithelial to mesenchymal transition. The Wnt/尾-Catenin-dependent expression of the reporter protein allowed quantification of dose- and time-dependent effects of teratogenic chems. In particular, valproic acid reduced reporter activity on day 7, whereas retinoic acid induced reporter activity on day 5 at concentrations comparable to the ones inhibiting the formation of functional cardiomyocytes, the classical read-out of the embryonic stem cell test (EST). In addition, the authors were also able to show distinct effects of teratogenic chems. on the Wnt/尾-Catenin-reporter compared with the previously described Bmp-reporter ESCs. Thus, different reporter cell lines provide complementary tools for the identification and anal. of potentially teratogenic compounds In the experiment, the researchers used many compounds, for example, 4-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline (cas: 1062368-24-4Recommanded Product: 4-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline).
4-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline (cas: 1062368-24-4) belongs to piperazine derivatives. Simple N-substituted piperazines have been found in many drug molecules. Piperazine is an anthelminthic especially useful in the treatment of partial intestinal obstruction caused by Ascaris worms, which is a condition primarily seen in children. Piperazine hydrate and piperazine citrate are the main anthelminthic piperazines.Recommanded Product: 4-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics