Determination of eight quinolones in milk using immunoaffinity microextraction in a packed syringe and liquid chromatography with fluorescence detection was written by Zhang, Xinda;Wang, Cuicui;Yang, Linyan;Zhang, Wei;Lin, Jing;Li, Cun. And the article was included in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences in 2017.COA of Formula: C19H20F3N3O3 This article mentions the following:
We have established a new, highly selective, and sensitive method for the determination of eight quinolones (QNs) in milk: danofloxacin, enrofloxacin, orbifloxacin, norfloxacin, ofloxacin, lomefloxacin, fleroxacin, and ciprofloxacin. The method uses immunoaffinity microextraction in a packed syringe and liquid chromatog. with fluorescence detection (IA-MEPS-LC-FLD). Traditionally, QN residues are determined by liquid-liquid extraction (LLE) and solid phase extraction (SPE) sample preparation techniques; however, these methods are time-consuming and require large quantities of organic solvents. We thus developed a novel immunoaffinity adsorbent combined with MEPS for QN residue anal. The syringe was filled with 0.2 g of microbeads bound with a QN monoclonal antibody using glutaraldehyde. The relevant parameters of the IA-MEPS method were optimized and discussed herein. Milk samples were extracted at a flow rate of 3.5 mL/min, 600 μL of methanol-and phosphate-buffered saline (9:1, volume/volume) was used for elution, and 200 μL of mobile phase was used for reconstitution after the sample was dried with nitrogen. Then, the sample was detected by LC-FLD. For the eight QNs, the limit of detection ranged from 0.05 to 0.1 ng/g, the limit of quantification ranged from 0.15 to 0.3 ng/g, and the intra- and inter-day precision were 3.2%-14.6% and 9.1%-15.8%, resp. The advantages of the IA-MEPS method includ simple operation, low cost and reduced organic solvent use. Moreover, the sample pretreatment is environmentally friendly because of the reduced solvent volume requirements. In the experiment, the researchers used many compounds, for example, 1-Cyclopropyl-7-(cis-3,5-dimethylpiperazin-1-yl)-5,6,8-trifluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (cas: 113617-63-3COA of Formula: C19H20F3N3O3).
1-Cyclopropyl-7-(cis-3,5-dimethylpiperazin-1-yl)-5,6,8-trifluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (cas: 113617-63-3) belongs to piperazine derivatives. The piperazine scaffold is often found in biologically active compounds in different therapeutic areas. These therapeutic areas include antifungals, antidepressants, antivirals, and serotonin receptor (5-HT) antagonists/agonists. Piperazine and its salts did not induce point mutations in a bacterial test. A series of mutagenicity studies in cells, both in vitro and in vivo, has been completed and showed no evidence of mutagenic effect.COA of Formula: C19H20F3N3O3
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics