Optimization for QuEChERS extraction of mycotoxins and veterinary drugs by response surface methodology for application to egg and milk was written by Zhou, Jian;Xu, Jiao-Jiao;Cong, Jin-Mi;Cai, Zeng-Xuan;Zhang, Jing-Shun;Wang, Jun-Lin;Ren, Yi-Ping. And the article was included in Journal of Chromatography A in 2018.Formula: C19H20F3N3O3 This article mentions the following:
A multiclass method was proposed for the simultaneous determination of various classes of veterinary drugs (n = 65), mycotoxins and metabolites (n = 39) in egg and milk by ultra-high performance liquid chromatog.-tandem mass spectrometry. The contaminants were extracted by QuEChERS-based strategy including salt-out partitioning and dispersive solid-phase extraction for cleanup further. With the aim of maximizing throughput and extraction efficiency, Plackett-Burman design was employed initially for screening significant variables. And response surface methodol. based on central composite design was conducted to achieve optimal conditions in details: 3.35% (volume/volume) of formic acid in acetonitrile, 1.2 g of NaCl, 0.5 g of anhydrous NaAc, 300 mg of C18 and 140 mg of primary secondary amine. Satisfactory anal. characteristics in validation, in aspects of accuracy (70%-105% for mycotoxins and quinolones, 55%-80% for sulfonamides and 40%-105% for other veterinary drugs), precision (inter-day RSDs < 14%) and sensitivity (LOQs ranged from 0.01 渭g/kg to 31 渭g/kg), were achieved under the optimized conditions. The matrix effects were evaluated and compensated by the use of matrix-matched calibration curves (R2 > 0.987). In practice, 45 eggs and 30 milk samples were investigated by the established method, of which pos. finding aflatoxin in milk and sterigmatocystin in eggs. In the experiment, the researchers used many compounds, for example, 1-Cyclopropyl-7-(cis-3,5-dimethylpiperazin-1-yl)-5,6,8-trifluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (cas: 113617-63-3Formula: C19H20F3N3O3).
1-Cyclopropyl-7-(cis-3,5-dimethylpiperazin-1-yl)-5,6,8-trifluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (cas: 113617-63-3) belongs to piperazine derivatives. Piperazine is a fairly basic compound and is an amine solvent. Piperazine is an anthelminthic especially useful in the treatment of partial intestinal obstruction caused by Ascaris worms, which is a condition primarily seen in children. Piperazine hydrate and piperazine citrate are the main anthelminthic piperazines.Formula: C19H20F3N3O3
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics