Ultrasound-assisted dispersive liquid-liquid microextraction for determination of enrofloxacin in surface waters was written by Dias, Reyla A. S.;Sousa, Eliane R.;Silva, Gilmar S.;Silva, Lanna K.;Freitas, Arlan S.;Lima, Diana L. D.;Sousa, Erika M. L.. And the article was included in Microchemical Journal in 2021.Synthetic Route of C20H17F2N3O3 This article mentions the following:
This work describes the development of an HPLC-FLD methodol. for the separation of five fluoroquinolones (ciprofloxacin, enrofloxacin, sarafloxacin, norfloxacin and levofloxacin) followed by optimization of the DLLME process for the clean-up and preconcentration of enrofloxacin in samples of seawater and river water. The mobile phase used for the chromatog. separation consisted of methanol: phosphate buffer (NaHPO4 H2O 0.04 M pH 3 with H3PO4 85%), gradient eluted at a ratio of 20:80 (v:v). The mobile phase flow was maintained at 1.2 mL min-1. For the ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME), the following conditions were used: 8 mL of sample with pH adjusted to 8, extraction solvent: 500渭L of chloroform, dispersive solvent: 500渭L of acetonitrile; samples were vortexed and sonicated for 2 min, each. The enrichment factor (EF) was 54.7 and the recovery was 70%, achieving a limit of detection (LOD) of 0.11渭g L-1. Repeatability and intermediate reproducibility presented values of relative standard deviation (RSD) lower than 2%. Finally, the optimized method was applied to the anal. of water and enrofloxacin was detected in both water samples with a concentration of 0.20渭g L-1 in the river and 0.12渭g L-1 in the seawater. However, recovery tests performed to evaluate the water matrixes’ effects on the extraction performance, presented recoveries of 72 卤 6.1 for river water and 27 卤 8.3 for seawater. These results demonstrate that hereby developed method is only suitable for water samples with a low salinity content. In the experiment, the researchers used many compounds, for example, 6-Fluoro-1-(4-fluorophenyl)-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid (cas: 98105-99-8Synthetic Route of C20H17F2N3O3).
6-Fluoro-1-(4-fluorophenyl)-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid (cas: 98105-99-8) belongs to piperazine derivatives. Simple N-substituted piperazines have been found in many drug molecules. Two common salts in the form of which piperazine is usually prepared for pharmaceutical or veterinary purposes are the citrate, 3C4H10N2.2C6H8O7 (i.e. containing 3 molecules of piperazine to 2 molecules of citric acid), and the adipate, C4H10N2.C6H10O4 (containing 1 molecule each of piperazine and adipic acid).Synthetic Route of C20H17F2N3O3
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics