Despras, Guillaume; Zamaleeva, Alsu I.; Dardevet, Lucie; Tisseyre, Celine; Magalhaes, Joao Gamelas; Garner, Charlotte; De Waard, Michel; Amigorena, Sebastian; Feltz, Anne; Mallet, Jean-Maurice; Collot, Mayeul published the artcile< H-Rubies, a new family of red emitting fluorescent pH sensors for living cells>, Product Details of C16H22N2O3, the main research area is H Rubies fluorescent hydrogen ion concentration sensor living cell.
Monitoring intracellular pH has drawn much attention due to its undeniably important function in cells. The widespread development of fluorescent imaging techniques makes pH sensitive fluorescent dyes valuable tools, especially red-emitting dyes which help to avoid the overcrowded green end of the spectral band. Herein, we present H-Rubies, a family of pH sensors based on a phenol moiety and a X-rhodamine fluorophore that display a bright red fluorescence upon acidification with pKa values spanning from 4 to 9. Slight structural modifications led to dramatic changes in their physicochem. properties and a relationship between their structures, their ability to form H-aggregates, and their apparent pKa was established. While mol. form H-Rubies can be used to monitor mitochondrial acidification of glioma cells, their functionalised forms were linked via click chem. to dextrans or microbeads containing a near IR Cy5 (Alexa-647) in order to provide ratiometric systems that were used to measure resp. the phagosomal and endosomal pH in macrophages (RAW 264.7 cells) using flow cytometry.
Chemical Science published new progress about Absorption spectra. 197638-83-8 belongs to class piperazines, and the molecular formula is C16H22N2O3, Product Details of C16H22N2O3.
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics