Hubicka, Urszula; Zmudzki, Pawel; Talik, Przemyslaw; Zuromska-Witek, Barbara; Krzek, Jan published an article in 2013, the title of the article was Photodegradation assessment of ciprofloxacin, moxifloxacin, norfloxacin and ofloxacin in the presence of excipients from tablets by UPLC-MS/MS and DSC.COA of Formula: C17H21ClFN3O4 And the article contains the following content:
Background: Ciprofloxacin (CIP), moxifloxacin (MOX), norfloxacin (NOR) and ofloxacin (OFL), are the antibacterial synthetic drugs, belonging to the fluoroquinolones group. Fluoroquinolones are compounds susceptible to photodegradation process, which may lead to reduction of their antibacterial activity and to induce phototoxicity as a side effect. This paper describes a simple, sensitive UPLC-MS/MS method for the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products. Results: Chromatog. separations were carried out using the Acquity UPLC BEH C18 column; (2.1 × 100 mm, 1.7 μm particle size). The column was maintained at 40°C and the following gradient was used: 0 min, 95% of eluent A and 5% of eluent B; 10 min, 0% of eluent A and 100% of eluent B, at a flow rate of 0.3 mL min-1. Eluent A: 0.1% (volume/volume) formic acid in water; eluent B: 0.1% (volume/volume) formic acid in acetonitrile. The method was validated and all the validation parameters were in the ranges acceptable by the guidelines for anal. method validation. The photodegradation of examined fluoroquinolones in solid phase in the presence of excipients followed kinetic of the first order reaction and depended upon the type of analyzed drugs and coexisting substances. Photodegradation process of analyzed drugs was confirmed by differential scanning calorimetry. In addition, the identification of degradation products was carried out by mass spectrometry. Conclusion: The developed UPLC-MS/MS method enables the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products and identification of photodegradation products. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).COA of Formula: C17H21ClFN3O4
The Article related to antibacterial agent excipient photodegradation uplc msms dsc, ciprofloxacin, differential scanning calorimetry, kinetic evaluation, moxifloxacin, norfloxacin, ofloxacin, photodegradation, tandem mass spectrometry, ultra performance liquid chromatography and other aspects.COA of Formula: C17H21ClFN3O4
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