Category: 86393-32-0

On June 30, 2022, Gunther, G.; Saathoff, E.; Rachow, A.; Ekandjo, H.; Diergaardt, A.; Marais, N.; Lange, C.; Nepolo, E. published an article.HPLC of Formula: 86393-32-0 The title of the article was Clinical evaluation of a line-probe assay for tuberculosis detection and drug-resistance prediction in Namibia. And the article contained the following:

Treatment of tuberculosis requires rapid information about Mycobacterium tuberculosis (Mtb) drug susceptibility to ensure effective therapy and optimal outcomes. At the tuberculosis referral hospital in Windhoek, Namibia, a country of high tuberculosis incidence, we evaluated the diagnostic accuracy of a line-probe-assay (LPA), GenID, for the mol. diagnosis of Mtb infection and drug resistance in patients with suspected tuberculosis (cohort 1) and confirmed rifampin (RIF)-resistant tuberculosis (cohort 2). GenID test results were compared to Xpert MTB/RIF and/or Mtb culture and antimicrobial suceptibilty testing. GenID LPA was applied to 79 and 55 samples from patients in cohort 1 and cohort 2, resp. The overall sensitivity of GenID LPA for the detection of Mtb DNA in sputum from patients with detectable and undetectable acid-fast bacilli by sputum smear microscopy was 93.3% (56/60; 95% confidence interval = 83.8-98.2) and 22.7% (5/22; 7.8-45.4). The sensitivity/specificity for the detection of drug resistance was 84.2% (32/38; 68.7-94.0)/100% (19/19; 82.4-100.0) for RIF, 89.7% (26/29; 72.6-97.8)/91.7% (22/24; 73.0-99.0) for isoniazid, and 85.7% (6/7; 42.1-99.6)/94.7% (18/19; 74.0-99.9) for fluoroquinolones; 23.6% of tests for second-line injectable resistance were invalid despite repeat testing. The diagnosis of tuberculosis by detection of Mtb DNA in sputum by GenID LPA depends strongly on the detection of acid-fast bacilli in sputum specimen. Prediction of drug resistance by GenID did not reach the World Health Organization (WHO) target product profile. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).HPLC of Formula: 86393-32-0

The Article related to tuberculosis drug resistance line probe assay namibia, xpert mtb/rif, diagnostics, multi-drug resistant, mycobacterium tuberculosis, mycobacterium tuberculosis detection, second-line resistance and other aspects.HPLC of Formula: 86393-32-0

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics

Hubicka, Urszula; Zmudzki, Pawel; Talik, Przemyslaw; Zuromska-Witek, Barbara; Krzek, Jan published an article in 2013, the title of the article was Photodegradation assessment of ciprofloxacin, moxifloxacin, norfloxacin and ofloxacin in the presence of excipients from tablets by UPLC-MS/MS and DSC.COA of Formula: C17H21ClFN3O4 And the article contains the following content:

Background: Ciprofloxacin (CIP), moxifloxacin (MOX), norfloxacin (NOR) and ofloxacin (OFL), are the antibacterial synthetic drugs, belonging to the fluoroquinolones group. Fluoroquinolones are compounds susceptible to photodegradation process, which may lead to reduction of their antibacterial activity and to induce phototoxicity as a side effect. This paper describes a simple, sensitive UPLC-MS/MS method for the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products. Results: Chromatog. separations were carried out using the Acquity UPLC BEH C18 column; (2.1 × 100 mm, 1.7 μm particle size). The column was maintained at 40°C and the following gradient was used: 0 min, 95% of eluent A and 5% of eluent B; 10 min, 0% of eluent A and 100% of eluent B, at a flow rate of 0.3 mL min-1. Eluent A: 0.1% (volume/volume) formic acid in water; eluent B: 0.1% (volume/volume) formic acid in acetonitrile. The method was validated and all the validation parameters were in the ranges acceptable by the guidelines for anal. method validation. The photodegradation of examined fluoroquinolones in solid phase in the presence of excipients followed kinetic of the first order reaction and depended upon the type of analyzed drugs and coexisting substances. Photodegradation process of analyzed drugs was confirmed by differential scanning calorimetry. In addition, the identification of degradation products was carried out by mass spectrometry. Conclusion: The developed UPLC-MS/MS method enables the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products and identification of photodegradation products. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).COA of Formula: C17H21ClFN3O4

The Article related to antibacterial agent excipient photodegradation uplc msms dsc, ciprofloxacin, differential scanning calorimetry, kinetic evaluation, moxifloxacin, norfloxacin, ofloxacin, photodegradation, tandem mass spectrometry, ultra performance liquid chromatography and other aspects.COA of Formula: C17H21ClFN3O4

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics

On June 30, 2013, Shou, Kai-jun; Li, Jie; Jin, Yi; Lv, Yan-wen published an article.Recommanded Product: 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate The title of the article was Design, synthesis, biological evaluation, and molecular docking studies of quinolone derivatives as potential antitumor topoisomerase I inhibitors. And the article contained the following:

A novel series of quinolone derivatives were designed and synthesized, and their biol. activities were evaluated as potential antitumor topoisomerase I (Top I) inhibitors. Among these compounds, one compound exhibited the most potent antitumor activities against multiple cancer cell lines. Docking simulation was performed to insert compound this compound into the crystal structure of DNA-Top I to determine the probable binding model. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).Recommanded Product: 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate

The Article related to phenylsulfonyl carbamidoyl quinolone preparation topoisomerase inhibitor structure activity relationship, topoisomerase phenylsulfonyl carbamidoyl quinolone docking antitumor drug design and other aspects.Recommanded Product: 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics

On August 30, 2020, Yu, Shihui; Yuan, Huiya; Chai, Guihong; Peng, Kuan; Zou, Peizhi; Li, Xuxi; Li, Jian; Zhou, Fanfan; Chan, Hak-Kim; Zhou, Qi Tony published an article.Product Details of 86393-32-0 The title of the article was Optimization of inhalable liposomal powder formulations and evaluation of their in vitro drug delivery behavior in Calu-3 human lung epithelial cells. And the article contained the following:

Inhalation therapy has advantages for the treatment of multidrug resistant bacterial lung infections with high drug concentrations at the infection sites in the airways and reduced systemic exposure. We have developed liposomal formulations for pulmonary delivery of synergistic ciprofloxacin (Cipro) and colistin (Col) as the potential candidate for treatment of lung infections caused by multidrug resistant Gram-neg. bacteria. This study aims to: (1) further optimize the powder formulation by adding drying stabilizers (polyvinyl pyrrolidone or poloxamer) to protect the liposomes during spray-freeze-drying; (2) evaluate the transport and cellular uptake of drugs in a human lung epithelial Calu-3 cell model. The liposomal powder formulations were produced using the ultrasonic spray-freeze-drying technique. The optimal formulation (F5) used mannitol (8% w/v) and sucrose (2% w/v) as the internal lyoprotectants. Adding external lyoprotectants/aerosolization enhancers (i.e. 8% w/v mannitol, 2% w/v sucrose and 1%, weight/weight PVP 10) produced the superior rehydrated EE values of ciprofloxacin and colistin (50.2 ± 0.9% for Cipro and 37.8 ± 1.2% for Col) as well as satisfactory aerosol performance (FPF: 34.2 ± 0.8% for Cipro and 33.6 ± 0.9% for Col). The cytotoxicity study indicated that F5 with the colistin concentration at 50μg/mL and ciprofloxacin at 200μg/mL was not cytotoxic to human lung epithelial Calu-3 cells. The intracellular uptake of ciprofloxacin was concentration-dependent in Calu-3 cells and the uptake of A-B was more than that of B-A for all samples (p < 0.05). This study demonstrates that co-delivery of ciprofloxacin and colistin in a single liposome can lower the transport capability of both drugs across the Calu-3 cell monolayer and their accumulation in the cells. These findings indicate that co-loaded liposomal powder of ciprofloxacin and colistin is a promising potential treatment for respiratory infections caused by multidrug resistant Gram-neg. bacteria. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).Product Details of 86393-32-0

The Article related to ciprofloxacin colistin sustained release liposome inhalation aerosol uptake, aerosol performance, calu-3 cell monolayer, ciprofloxacin, colistin, drug transport, liposomal powder and other aspects.Product Details of 86393-32-0

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics

On February 6, 2003, Stankovic, Slobodan; Mitov, Slobodan; Stanojevic, Caslav published a patent.Synthetic Route of 86393-32-0 The title of the patent was A process for synthesis of antibiotic fluoroquinolonic acid derivatives. And the patent contained the following:

A simple and convenient procedure for obtaining antibiotics of fluoroquinolonic derivatives of general formula (I; where R, R2 = H, C1-4 alkyl; R1 = C1-4 alkyl, cycloalkyl such as cyclopropyl), and/or salts and hydrates thereof, in particular ciprofloxacin and norfloxacin, and is developed by amination of piperazine or piperazine derivatives (II; R = same as above) with the 6-fluoro-7-chloro-1,4-dihydro-4-oxoquinoline-3-carboxylic acid derivative of general formula (III; R1 , R2 = same as above) in an inert solvent of pharmacopoeic purity, at risen temperature The process is characterized in lower reaction temperature, atm. pressure reaction, tech. simplicity of the procedure of purification by conversion and isolation in the form of pharmaceutically acceptable salts, increased yields, reducing cost on the procedure for industrial use, as well as pharmacopoeic purity of the product, enabled their use as the antibiotics in human and veterinary medicine. Thus, a mixture of 49.25 g 7-chloro-1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxoquinoline-3-carboxylic acid, 72.25 g piperazine, and 250 cm3 of DMSO was heated for 1.5 to 2 h at 140°, cooled to 70°, treated with 985 cm3 distilled water, and then treated with 62.5 cm3 concentrated HCl with stirring and cooling. Formed suspension was filtered and the precipitate was rinsed with distilled water, suspended in water, dissolved by addition of 2 mol/dm3 HCl, treated with active charcoal, heated with stirring at 50°, and filtered. To the filtrate was added 2 mol/dm3 NaOH with stirring and cooling and the formed suspension was filtered. The precipitate was rinsed with distilled water, suspended in water with stirring, treated with 60 cm3 2 mol/dm3 HCl, heated for 30 min at 75-80°, and added to 1,750 cm3 absolute ethanol. The mixture was cooled to 0-5° and filtered, and the precipitate was rinsed three times with 30 cm3 absolute ethanol each time, and dried in vacuum drier at 80° to give 49.46 g ciprofloxacin hydrochloride monohydrate (1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-piperazinoquinoline-3-carboxylic acid hydrochloride monohydrate) as white crystals having m.p. 308-310° (decomposition) in 73% yield. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).Synthetic Route of 86393-32-0

The Article related to fluoroquinolonic acid preparation antibiotic, ciprofloxacin hydrochloride monohydrate preparation antibiotic, fluoropiperazinodihydrooxoquinolinecarboxylic acid preparation antibiotic, norfloxacin preparation antibiotic and other aspects.Synthetic Route of 86393-32-0

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics

On August 10, 2022, Wang, Linqiong; Li, Yi; Zhao, Zhe; Zhu, Mengjie; Hu, Tong published an article.Reference of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate The title of the article was Tidal flat aquaculture pollution governs sedimentary antibiotic resistance gene profiles but not bacterial community based on metagenomic data. And the article contained the following:

Coastal tidal flats are intersection zones between terrestrial and marine environments and are considered repositories of pollutants from anthropogenic activities (e.g., fishery and aquaculture). Specifically, the prevalence of antibiotics and antibiotic resistance genes (ARGs) in coastal aquaculture environments pose critical threats to estuarine ecosystems. However, the contribution of aquaculture to the occurrence and abundance of ARGs and community assemblies has not been fully explored in tidal flat zones. Thus, we investigated ARGs profiles, ARG-carrying host bacteria, and their associate microbial community in the Dongtai and Sheyang tidal flat aquaculture regions of Jiangsu, China using metagenomic assembly methods. The antibiotic concentrations in the sediment samples ranged from nd to 35.50 ng/g dw, and the antibiotic pollution in the Dongtai tidal flat was more severe than in the Sheyang tidal flats. Metagenomic assembly indicated that a total of 247 ARG subtypes associated with ARG 33 types were characterized across all samples and their abundance in the Dongtai region exceeded that in the Sheyang region. Meanwhile, 21 bacteria in the tidal flat aquaculture were identified as ARG-carrying pathogens, including Escherichia coli, Vibrio fluvialis, and Staphylococcus aureus. Using neutral and null modeling anal. to determine the community ecol. processes, the results revealed bacterial and ARG communities were generally dominated by stochastic and deterministic processes, resp. The above results suggested that aquaculture pollution was contributed to shape ARG profiles in tidal flats. The observed deterministic processes affecting the ARG community in tidal flat aquaculture also provides an effective foundation to control the risks of environmental antibiotic resistance through reducing aquaculture antibiotic usage. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).Reference of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate

The Article related to antibiotic resistance gene bacterial community metagenomic data aquaculture pollution, arg host bacteria, antibiotic resistance genes, ecological processes, metagenomic assembly, tidal flat aquaculture and other aspects.Reference of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics

On June 30, 2022, Gunther, G.; Saathoff, E.; Rachow, A.; Ekandjo, H.; Diergaardt, A.; Marais, N.; Lange, C.; Nepolo, E. published an article.HPLC of Formula: 86393-32-0 The title of the article was Clinical evaluation of a line-probe assay for tuberculosis detection and drug-resistance prediction in Namibia. And the article contained the following:

Treatment of tuberculosis requires rapid information about Mycobacterium tuberculosis (Mtb) drug susceptibility to ensure effective therapy and optimal outcomes. At the tuberculosis referral hospital in Windhoek, Namibia, a country of high tuberculosis incidence, we evaluated the diagnostic accuracy of a line-probe-assay (LPA), GenID, for the mol. diagnosis of Mtb infection and drug resistance in patients with suspected tuberculosis (cohort 1) and confirmed rifampin (RIF)-resistant tuberculosis (cohort 2). GenID test results were compared to Xpert MTB/RIF and/or Mtb culture and antimicrobial suceptibilty testing. GenID LPA was applied to 79 and 55 samples from patients in cohort 1 and cohort 2, resp. The overall sensitivity of GenID LPA for the detection of Mtb DNA in sputum from patients with detectable and undetectable acid-fast bacilli by sputum smear microscopy was 93.3% (56/60; 95% confidence interval = 83.8-98.2) and 22.7% (5/22; 7.8-45.4). The sensitivity/specificity for the detection of drug resistance was 84.2% (32/38; 68.7-94.0)/100% (19/19; 82.4-100.0) for RIF, 89.7% (26/29; 72.6-97.8)/91.7% (22/24; 73.0-99.0) for isoniazid, and 85.7% (6/7; 42.1-99.6)/94.7% (18/19; 74.0-99.9) for fluoroquinolones; 23.6% of tests for second-line injectable resistance were invalid despite repeat testing. The diagnosis of tuberculosis by detection of Mtb DNA in sputum by GenID LPA depends strongly on the detection of acid-fast bacilli in sputum specimen. Prediction of drug resistance by GenID did not reach the World Health Organization (WHO) target product profile. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).HPLC of Formula: 86393-32-0

The Article related to tuberculosis drug resistance line probe assay namibia, xpert mtb/rif, diagnostics, multi-drug resistant, mycobacterium tuberculosis, mycobacterium tuberculosis detection, second-line resistance and other aspects.HPLC of Formula: 86393-32-0

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics

Hubicka, Urszula; Zmudzki, Pawel; Talik, Przemyslaw; Zuromska-Witek, Barbara; Krzek, Jan published an article in 2013, the title of the article was Photodegradation assessment of ciprofloxacin, moxifloxacin, norfloxacin and ofloxacin in the presence of excipients from tablets by UPLC-MS/MS and DSC.COA of Formula: C17H21ClFN3O4 And the article contains the following content:

Background: Ciprofloxacin (CIP), moxifloxacin (MOX), norfloxacin (NOR) and ofloxacin (OFL), are the antibacterial synthetic drugs, belonging to the fluoroquinolones group. Fluoroquinolones are compounds susceptible to photodegradation process, which may lead to reduction of their antibacterial activity and to induce phototoxicity as a side effect. This paper describes a simple, sensitive UPLC-MS/MS method for the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products. Results: Chromatog. separations were carried out using the Acquity UPLC BEH C18 column; (2.1 × 100 mm, 1.7 μm particle size). The column was maintained at 40°C and the following gradient was used: 0 min, 95% of eluent A and 5% of eluent B; 10 min, 0% of eluent A and 100% of eluent B, at a flow rate of 0.3 mL min-1. Eluent A: 0.1% (volume/volume) formic acid in water; eluent B: 0.1% (volume/volume) formic acid in acetonitrile. The method was validated and all the validation parameters were in the ranges acceptable by the guidelines for anal. method validation. The photodegradation of examined fluoroquinolones in solid phase in the presence of excipients followed kinetic of the first order reaction and depended upon the type of analyzed drugs and coexisting substances. Photodegradation process of analyzed drugs was confirmed by differential scanning calorimetry. In addition, the identification of degradation products was carried out by mass spectrometry. Conclusion: The developed UPLC-MS/MS method enables the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products and identification of photodegradation products. The experimental process involved the reaction of 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid hydrochloride hydrate(cas: 86393-32-0).COA of Formula: C17H21ClFN3O4

The Article related to antibacterial agent excipient photodegradation uplc msms dsc, ciprofloxacin, differential scanning calorimetry, kinetic evaluation, moxifloxacin, norfloxacin, ofloxacin, photodegradation, tandem mass spectrometry, ultra performance liquid chromatography and other aspects.COA of Formula: C17H21ClFN3O4

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics